Learn about pas staining, its protocol, applications in pathology, and troubleshooting tips for accurate results in diagnostic and research settings. A total of 25 out of 25 aml cases and 28 out of 32 cases of all were correctly diagnosed based on morphology and cytochemical staining. Periodic acid schiff (pas) stain performed on a bone marrow aspirate smear involved by acute erythroid leukemia (formerly known as pure erythroid leukemia)
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Note the abnormal (chunky globular) pattern of staining in the erythroblasts.
Monocytes are faintly positive while lymphocytes and red blood cells lack the enzyme
This stain is particularly useful in a setting of acute leukaemia, where positive staining indicates that the leukaemic cells are of myeloid origin. Cytochemical analysis of leukaemia using pas and mpo rendered the diagnosis in 92.9% of acute leukaemia cases in our study
